2VQX
Precursor of Protealysin, Metalloproteinase from Serratia proteamaculans.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | KURCHATOV SNC BEAMLINE K4.4 |
Synchrotron site | KURCHATOV SNC |
Beamline | K4.4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-19 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.760, 78.650, 59.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.630 - 1.821 |
R-factor | 0.17141 |
Rwork | 0.170 |
R-free | 0.20205 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1npc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.039 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | BALBES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.600 | 2.000 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.030 | 0.090 |
Number of reflections | 28551 | |
<I/σ(I)> | 26.9 | 11.07 |
Completeness [%] | 90.9 | 78.6 |
Redundancy | 2.82 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | THE HANGING-DROP VAPOR DIFFUSION METHOD AGAINST A PRECIPITANT SOLUTION OF 15% PEG MME 2000, 0.2M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE PH 4.6. DROPLETS CONTAINED 3 MKL OF 10 MG/ML PROTEIN SOLUTION AND 1.5 MKL PRECIPITANT SOLUTION WITH 0.06% BETA-OCTIL-D-GLUCOPYRANOSIDE IN THE SAME BUFFER |