2VQS
Structural Studies of Nucleoside Analog and Feedback Inhibitor Binding to Drosophila Melanogaster Multisubstrate Deoxyribonucleoside Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-02 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 137.644, 112.828, 69.721 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.244 |
Rwork | 0.242 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j90 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.398 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.090 | 0.460 |
Number of reflections | 19428 | |
<I/σ(I)> | 7.5 | 1.6 |
Completeness [%] | 83.5 | 87.2 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |