2VQD
Crystal Structure of Biotin Carboxylase from Pseudomonas aeruginosa complexed with AMPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Collection date | 2007-05-01 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.227, 126.786, 49.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.370 - 2.410 |
| R-factor | 0.182 |
| Rwork | 0.178 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dv2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.350 |
| Number of reflections | 15777 | |
| <I/σ(I)> | 22.9 | 2.86 |
| Completeness [%] | 96.0 | 83.9 |
| Redundancy | 5.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M HEPES (PH 7.0), 0.2M MGCL2, 15-20% PEG3350 |
