2VQC

Structure of a DNA binding winged-helix protein, F-112, from Sulfolobus Spindle-shaped Virus 1.

Replaces:  2CMX

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-16
Detector	MARRESEARCH
Wavelength(s)	0.891940, 0.979180, 0.979320
Spacegroup name	H 3
Unit cell lengths	87.716, 87.716, 34.491
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.530 - 2.300
R-factor	0.175
Rwork	0.173
R-free	0.19900
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	0.923
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.110
Number of reflections	4411
<I/σ(I)>	20.6	12.3
Completeness [%]	99.9	100
Redundancy	7.7	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6		HANGING DROP VAPOR DIFFUSION IN 0.1M MES (PH 6.0). QIUCK SOAK IN ML CONTAINING 25% GLYCEROL AS A CRYOPROTECTANT.