2VQB
Bacterial flavin-containing monooxygenase in complex with NADP: soaking in aerated solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 61 |
| Unit cell lengths | 218.665, 218.665, 130.676 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.720 - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vq7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.432 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.430 |
| Number of reflections | 86940 | |
| <I/σ(I)> | 13.1 | 2.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% W/V PEG4000, 100 MM NA-HEPES, 1 MM NADP PH 7.5 |
