2VQ9
RNASE ZF-3E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-05 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 43.168, 60.964, 115.229 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.340 - 1.850 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vq8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.548 |
| Data reduction software | HKL (SUITE) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.120 | 0.250 |
| Number of reflections | 13420 | |
| <I/σ(I)> | 14.5 | 5.1 |
| Completeness [%] | 98.8 | 90.6 |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 25% PEG 4000, 0.2M LITHIUM CHLORIDE, 0.1M TRIS-HCL PH 8.5 |
