2VQ8
RNASE ZF-1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.155, 39.426, 46.126 |
| Unit cell angles | 90.00, 98.80, 90.00 |
Refinement procedure
| Resolution | 23.240 - 1.350 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.284 |
| Data reduction software | HKL (SUITE) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.040 | 0.310 |
| Number of reflections | 27012 | |
| <I/σ(I)> | 22.8 | 2 |
| Completeness [%] | 93.7 | 61.8 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 25% PEG 4000, 0.2M AMMONIUM SULPHATE, 0.1M TRIS-HCL PH 8.0 |
