2VQ7
Bacterial flavin-containing monooxygenase in complex with NADP: native data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 219.589, 219.589, 133.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 188.980 - 2.600 |
| R-factor | 0.249 |
| Rwork | 0.248 |
| R-free | 0.26900 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.422 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 0.450 |
| Number of reflections | 110675 | |
| <I/σ(I)> | 8 | 2.3 |
| Completeness [%] | 97.3 | 86.1 |
| Redundancy | 3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% W/V PEG4000, 100 MM SODIUM HEPES PH 7.5, 1 MM NADP |
