2VQ4
Carbohydrate-binding of the starch binding domain of Rhizopus oryzae glucoamylase in complex with beta-cyclodextrin and maltoheptaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2008-02-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.635, 33.894, 93.389 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.250 |
| R-factor | 0.147 |
| Rwork | 0.146 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.382 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.070 | 0.290 |
| Number of reflections | 23978 | |
| <I/σ(I)> | 31.5 | 6.7 |
| Completeness [%] | 98.5 | 93.9 |
| Redundancy | 11.6 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG4000 |
