2VQ2
Crystal structure of PilW, widely conserved type IV pilus biogenesis factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 87.446, 87.446, 117.102 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.810 - 1.540 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.18300 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.174 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.060 | 0.500 |
| Number of reflections | 71188 | |
| <I/σ(I)> | 37 | 7.1 |
| Completeness [%] | 96.0 | 88.3 |
| Redundancy | 23.15 | 21.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | HANGING-DROP VAPOR DIFFUSION METHOD, 20C 100MM TRIS HCL PH8.5, 800MM LI2SO4, 10MM NICL2 |
