2VPR
Tet repressor class H in complex with 5a,6- anhydrotetracycline-Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-24 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.361, 108.744, 68.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.490 |
| R-factor | 0.209 |
| Rwork | 0.201 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tct |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.576 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.510 |
| Number of reflections | 7669 | |
| <I/σ(I)> | 9.6 | 2.8 |
| Completeness [%] | 98.0 | 69 |
| Redundancy | 4.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 3.31 MG/ML TETR(H), 100 UL 2 MM ANTC,100 UL TETR(H),0.5 UL 3 M MGCL2, 20 % PEG1500, 0.1 M NAHEPES PH 7.5, 2 UL RESERVOIR LSG PLUS 2 UL PROTEIN |
