2VPN
High-resolution structure of the periplasmic ectoine-binding protein from TeaABC TRAP-transporter of Halomonas elongata
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-06 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 48.300, 52.200, 63.500 |
Unit cell angles | 80.80, 85.80, 77.90 |
Refinement procedure
Resolution | 20.000 - 1.550 |
R-factor | 0.159 |
Rwork | 0.158 |
R-free | 0.20400 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.291 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.650 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.010 | 0.060 |
Number of reflections | 81024 | |
<I/σ(I)> | 9.2 | 2.3 |
Completeness [%] | 93.3 | 92.3 |
Redundancy | 3 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES PH 7.5, 0.1 M MGCL2 AND 25.5 % PEG 3350 |