2VPH
Crystal structure of the human protein tyrosine phosphatase, non- receptor type 4, PDZ domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-26 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.489, 71.015, 71.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 1.900 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2OCS 2he2 1qav |
RMSD bond length | 0.013 |
RMSD bond angle | 1.448 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.100 | 0.590 |
Number of reflections | 15306 | |
<I/σ(I)> | 10.9 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 20% PEG 3350, 0.20M (NH4)2H(CIT), PH 5 |