2VPF

VASCULAR ENDOTHELIAL GROWTH FACTOR REFINED TO 1.93 ANGSTROMS RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1996-03
Detector	PRINCETON 2K
Spacegroup name	P 1
Unit cell lengths	45.470, 68.470, 85.820
Unit cell angles	105.44, 93.71, 101.49

Refinement procedure

Resolution	16.000 - 1.930
R-factor	0.209 *
Rwork	0.209
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vpf
RMSD bond length	0.011
RMSD bond angle	0.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.044	0.127 *
Total number of observations	180803 *
Number of reflections	72050
<I/σ(I)>	9.4
Completeness [%]	94.0	81 *
Redundancy	2.5	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *		HANGING DROP, CRYSTALLIZED FROM 14 % PEG3350, 10% ISOPROPANOL, 0.2 M AMMONIUM ACETATE PH 5.6, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		0.4 (M)
4	1	reservoir	PEG3350	14 (%)
5	1	reservoir	isopropanol	10 (%)
6	1	reservoir	ammonium sulfate	0.2 (M)