2VPF
VASCULAR ENDOTHELIAL GROWTH FACTOR REFINED TO 1.93 ANGSTROMS RESOLUTION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-03 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.470, 68.470, 85.820 |
| Unit cell angles | 105.44, 93.71, 101.49 |
Refinement procedure
| Resolution | 16.000 - 1.930 |
| R-factor | 0.209 * |
| Rwork | 0.209 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vpf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.044 | 0.127 * |
| Total number of observations | 180803 * | |
| Number of reflections | 72050 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 94.0 | 81 * |
| Redundancy | 2.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | HANGING DROP, CRYSTALLIZED FROM 14 % PEG3350, 10% ISOPROPANOL, 0.2 M AMMONIUM ACETATE PH 5.6, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 0.4 (M) | ||
| 4 | 1 | reservoir | PEG3350 | 14 (%) | |
| 5 | 1 | reservoir | isopropanol | 10 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
