2VOX
An oxidized tryptophan facilitates copper-binding in Methylococcus capsulatus secreted protein MopE. The structure of mercury soaked MopE to 1.9AA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.939, 101.282, 54.816 |
| Unit cell angles | 90.00, 98.32, 90.00 |
Refinement procedure
| Resolution | 41.130 - 1.900 |
| R-factor | 0.222 |
| Rwork | 0.221 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.347 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.100 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.550 |
| Number of reflections | 27480 | |
| <I/σ(I)> | 8.4 | 1.3 |
| Completeness [%] | 98.2 | 97.3 |
| Redundancy | 13.4 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 36-45% AMMONIUM SULPHATE, 0.1 M BISTRIS PH 7.0-7.75 |
