2VOW
An oxidized tryptophan facilitates copper-binding in Methylococcus capsulatus secreted protein MopE. The structure of recombinant MopE to 1.65AA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.280, 100.670, 54.650 |
| Unit cell angles | 90.00, 97.56, 90.00 |
Refinement procedure
| Resolution | 36.000 - 1.650 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vox |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.348 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.900 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.050 | 0.330 |
| Number of reflections | 41358 | |
| <I/σ(I)> | 7.5 | 2.3 |
| Completeness [%] | 98.4 | 89.9 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 36-46% AMMONIUM SULPHATE, 0.1M BISTRIS PH6.5-7.0,1.5%GLYCEROL |
