2VNZ
Crystal structure of dithinonite reduced soybean ascorbate peroxidase mutant W41A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 81.780, 81.780, 75.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.180 - 1.300 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ggn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.117 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.830 | 1.390 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 63112 | |
| <I/σ(I)> | 21.6 | 4.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.29 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.3 | pH 8.3 |
