2VN4

Glycoside Hydrolase Family 15 Glucoamylase from Hypocrea jecorina

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-5
Synchrotron site	MAX II
Beamline	I911-5
Temperature [K]	295
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	52.185, 99.232, 121.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.700 - 1.850
R-factor	0.15559
Rwork	0.154
R-free	0.18211
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1glm
RMSD bond length	0.010
RMSD bond angle	1.204
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.700	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.090	0.450
Number of reflections	56858
<I/σ(I)>	20.2	5.3
Completeness [%]	98.3	97.7
Redundancy	8.5	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		25% PEG 3350, 0.20M AMMONIUM ACETATE, 0.10M BIS-TRIS PH 5.5