2VN2

Crystal structure of the N-terminal domain of DnaD protein from Geobacillus kaustophilus HTA426

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Wavelength(s)	0.9794, 0.9795, 0.9643
Spacegroup name	F 2 2 2
Unit cell lengths	116.514, 124.707, 157.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.490 - 2.300
R-factor	0.217
Rwork	0.217
R-free	0.22300
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.034
RMSD bond angle	2.600
Data reduction software	Blu-Ice (CONTROL SOFTWARE)
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.490	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.110	0.370
Number of reflections	46920
<I/σ(I)>	22.59	9.2
Completeness [%]	95.4	100
Redundancy	12.3	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1