2VMK
Crystal Structure of E. coli RNase E Apoprotein - Catalytic Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-03 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 73.241, 75.571, 109.370 |
Unit cell angles | 94.95, 102.03, 91.77 |
Refinement procedure
Resolution | 47.840 - 3.300 |
R-factor | 0.268 |
Rwork | 0.267 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bx2 |
RMSD bond length | 0.025 |
RMSD bond angle | 0.950 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.340 |
High resolution limit [Å] | 3.220 | 3.220 |
Rmerge | 0.110 | 0.460 |
Number of reflections | 34917 | |
<I/σ(I)> | 11.1 | 1.7 |
Completeness [%] | 91.4 | 67.5 |
Redundancy | 3.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.5 |