2VMG
The structure of CBM51 from Clostridium perfringens GH95 in complex with methyl-galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 113 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.475, 47.948, 38.704 |
Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.233 |
Rwork | 0.230 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.932 |
Data reduction software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.077 | 0.300 |
Number of reflections | 10010 | |
<I/σ(I)> | 8.6 | 2.5 |
Completeness [%] | 96.4 | 93.2 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 |