2VMD
Structure of the complex of discoidin II from Dictyostelium discoideum with beta-methyl-galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-06 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 81.140, 81.140, 259.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.740 - 1.900 |
| R-factor | 0.181 |
| Rwork | 0.178 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vm9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.494 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.700 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.310 |
| Number of reflections | 26194 | |
| <I/σ(I)> | 8.7 | 2.35 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 4.4 | 4.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 25 % PEG550 MME, pH 7.5 |
