2VMB
The three-dimensional structure of the cytoplasmic domains of EpsF from the Type 2 Secretion System of Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-29 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.197, 53.832, 89.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.080 - 1.950 |
R-factor | 0.192 |
Rwork | 0.189 |
R-free | 0.25100 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.130 |
Data scaling software | CrystalClear |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.070 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.050 | 0.140 |
Number of reflections | 16364 | |
<I/σ(I)> | 36 | 10.2 |
Completeness [%] | 91.7 | 59.1 |
Redundancy | 12.96 | 8.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 1UL PROTEIN, 1UL RESERVOIR: 12.5% PEG 400, 200MM CAOAC2, 100MM MES PH 7.0 |