2VM2
Crystal structure of barley thioredoxin h isoform 1 crystallized using PEG as precipitant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.726, 33.663, 132.202 |
| Unit cell angles | 90.00, 112.48, 90.00 |
Refinement procedure
| Resolution | 20.360 - 1.800 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ep7 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.342 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.600 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.350 |
| Number of reflections | 46412 | |
| <I/σ(I)> | 6.5 | 2.1 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
