2VLV

Crystal structure of barley thioredoxin h isoform 2 in partially radiation-reduced state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	49.249, 38.650, 57.294
Unit cell angles	90.00, 105.69, 90.00

Refinement procedure

Resolution	24.300 - 1.700
R-factor	0.206
Rwork	0.205
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.880
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.300	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.030	0.270
Number of reflections	21980
<I/σ(I)>	12.3	2.7
Completeness [%]	95.2	81.7
Redundancy	3.4	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1