2VLU

Crystal structure of barley thioredoxin h isoform 2 in partially radiation-reduced state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	49.254, 38.601, 57.282
Unit cell angles	90.00, 105.81, 90.00

Refinement procedure

Resolution	24.590 - 1.700
R-factor	0.205
Rwork	0.204
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.865
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.600	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.030	0.200
Number of reflections	21964
<I/σ(I)>	13.5	3.6
Completeness [%]	95.3	82.5
Redundancy	3.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1