2VLT

Crystal structure of barley thioredoxin h isoform 2 in the oxidized state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	47.256, 38.370, 56.554
Unit cell angles	90.00, 109.77, 90.00

Refinement procedure

Resolution	53.220 - 2.000
R-factor	0.19
Rwork	0.186
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.807
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.600	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.150	0.260
Number of reflections	12288
<I/σ(I)>	2.9	2.3
Completeness [%]	92.3	94.7
Redundancy	2.5	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1