2VLQ

F86A mutant of E9 DNase domain in complex with Im9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	110
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	36.058, 77.490, 41.807
Unit cell angles	90.00, 100.21, 90.00

Refinement procedure

Resolution	41.170 - 1.600
R-factor	0.166
Rwork	0.164
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1emv
RMSD bond length	0.018
RMSD bond angle	1.707
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.170	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.070	0.340
Number of reflections	29183
<I/σ(I)>	17.1	4.1
Completeness [%]	97.7	85.4
Redundancy	3.9	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				2.9 M AMMONIUM SULPHATE AND 100 MM BIS-TRIS PH 5.5