2VLP
R54A mutant of E9 DNase domain in complex with Im9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 110 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.285, 77.920, 41.402 |
Unit cell angles | 90.00, 100.07, 90.00 |
Refinement procedure
Resolution | 40.820 - 2.000 |
R-factor | 0.169 |
Rwork | 0.166 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1emv |
RMSD bond length | 0.018 |
RMSD bond angle | 1.574 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.340 |
Number of reflections | 15357 | |
<I/σ(I)> | 18 | 5.8 |
Completeness [%] | 98.4 | 98.4 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2.9 M AMMONIUM SULPHATE AND 100 MM BIS-TRIS PH 5.5 |