2VL3
Oxidized and reduced forms of human peroxiredoxin 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-07 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.350, 102.120, 146.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.938 - 1.830 |
| R-factor | 0.238 |
| Rwork | 0.235 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hd2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.208 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 1.950 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 51613 | |
| <I/σ(I)> | 12.3 | 3.37 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | NACL 0.1 M TRIS PH 8 0.1 M PEG 3350 20% |
