2VL2
Oxidized and reduced forms of human peroxiredoxin 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-07 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.067, 102.016, 146.643 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.500 - 1.925 |
| R-factor | 0.2074 |
| Rwork | 0.205 |
| R-free | 0.25490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hd2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.018 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.500 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.030 | 0.490 |
| Number of reflections | 42806 | |
| <I/σ(I)> | 24.6 | 2.7 |
| Completeness [%] | 94.6 | 93.3 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | NACL 0.1M TRIS PH 8 0.1M PEG 3350 20% |
