2VKL
X-ray crystal structure of the intracellular Chorismate mutase from Mycobactrerium Tuberculosis in complex with malate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-04 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.269, 62.269, 37.483 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.980 - 1.650 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.760 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.050 | 0.680 |
| Number of reflections | 9314 | |
| <I/σ(I)> | 23.81 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.62 | 6.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1M MMT BUFFER PH 9, 25% W/V PEG 1500 |
