2VJU
Crystal structure of the IS608 transposase in complex with the complete Right end 35-mer DNA and manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2006-11-20 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.550, 95.705, 98.561 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.040 - 2.400 |
Rwork | 0.186 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vih |
RMSD bond length | 0.008 |
RMSD bond angle | 2.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNX (2005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.100 | 0.420 |
Number of reflections | 22916 | |
<I/σ(I)> | 13.7 | 2.7 |
Completeness [%] | 98.3 | 94.9 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 20-25% PEG 3350, 0.1 M MES PH 5.5, AND 0.1 M AMMONIUM ACETATE |