2VJR
The structure of phycocyanin from Gloeobacter violaceus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 117.538, 117.538, 116.163 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 101.530 - 2.600 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jbo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.982 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 2.510 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.080 | 0.410 |
| Number of reflections | 16967 | |
| <I/σ(I)> | 34.42 | 5.56 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 21.11 | 20.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 33.3 MM LITHIUM SULFATE, 17 MM TRIS HYDROCHLORIDE PH 8.5, 5% W/V PEG 4K, MIXED WITH AN EQUAL VOLUME OF PROTEIN SOLUTION. HANGING DROP VAPOUR DIFFUSION. |
