2VJQ
Formyl-CoA transferase mutant variant W48Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-28 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.155, 98.917, 152.527 |
| Unit cell angles | 90.00, 135.27, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.113 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.080 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.410 |
| Number of reflections | 190053 | |
| <I/σ(I)> | 12.4 | 2.3 |
| Completeness [%] | 92.0 | 68.7 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
