2VJL
Formyl-CoA transferase with aspartyl-CoA thioester intermediate derived from formyl-CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-04 |
Detector | ADSC CCD |
Spacegroup name | I 4 |
Unit cell lengths | 151.765, 151.765, 100.082 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p5h |
RMSD bond length | 0.007 |
RMSD bond angle | 1.075 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.110 | 0.390 |
Number of reflections | 75233 | |
<I/σ(I)> | 8.7 | 1.8 |
Completeness [%] | 98.3 | 94.3 |
Redundancy | 3.1 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |