2VJI
Tailspike protein of E.coli bacteriophage HK620
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-08 |
Detector | ADSC CCD |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 74.216, 74.216, 175.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.380 |
R-factor | 0.151 |
Rwork | 0.150 |
R-free | 0.18000 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.009 |
RMSD bond angle | 1.499 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE (RESOLVE V2.09) |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.430 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.060 | 0.260 |
Number of reflections | 115675 | |
<I/σ(I)> | 12.7 | 5.1 |
Completeness [%] | 97.5 | 90.2 |
Redundancy | 4.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3 | VAPOR DIFFUSION,HANGING DROP: PROTEIN CONCENTRATION 3MG/ML,5% PEG1500,PH3.0. RESERVOIR: 10% PEG 1500, PH 3.0. DROPLET 3 MICRO LITER: 3 MICRO LITER. CRYO CONDITION:30% PEG1500. |