2VJH
The structure of Phycoerythrin from Gloeobacter violaceus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 155.829, 155.829, 155.829 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.230 - 2.200 |
| R-factor | 0.155 |
| Rwork | 0.153 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b8d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.328 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.990 | 2.210 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.130 | 0.340 |
| Number of reflections | 64149 | |
| <I/σ(I)> | 19.53 | 4.28 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.06 | 9.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% W/V PEG 8K, 0.33 M SODIUM CACODYLATE, 0.67 M MAGNESIUM CHLORIDE |
