2VJ4
Methylated Shigella flexneri MxiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2007-06-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 2 2 2 |
| Unit cell lengths | 83.480, 83.450, 117.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.000 - 2.500 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vix |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.935 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | TNT (5.13.1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.520 |
| Number of reflections | 28754 | |
| <I/σ(I)> | 14.2 | 2.7 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.0 M SUCCINIC ACID, 0.1 M HEPES PH 7.0, 1% W/V PEGMME 2000 |
