2VJ2
Human Jagged-1, domains DSL and EGFs1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.000, 102.300, 62.700 |
| Unit cell angles | 90.00, 114.30, 90.00 |
Refinement procedure
| Resolution | 48.500 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.23900 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.716 |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | TNT (5.6.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.100 | 2.740 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.420 |
| Number of reflections | 11962 | |
| <I/σ(I)> | 13.1 | 3.3 |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 8.5% (W/V) PEG4000, 100MM IMIDAZOLE -MALATE, PH7.0 |
