2VIM
X-ray structure of Fasciola hepatica thioredoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.666, 34.586, 40.381 |
| Unit cell angles | 90.00, 106.63, 90.00 |
Refinement procedure
| Resolution | 38.690 - 1.380 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fa4 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.679 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | Babel |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.690 | 1.420 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.070 | 0.490 |
| Number of reflections | 16069 | |
| <I/σ(I)> | 10.75 | 0.87 |
| Completeness [%] | 89.6 | 56.3 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-HCL PH 8.5, 2 M AMMONIUM SULFATE. |
