2VIH

CRYSTAL STRUCTURE OF THE IS608 TRANSPOSASE IN COMPLEX WITH Left END 26-MER DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	95
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	73.482, 50.287, 115.554
Unit cell angles	90.00, 105.74, 90.00

Refinement procedure

Resolution	29.970 - 2.100
R-factor	0.225
Rwork	0.225
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a6o
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.120	0.450
Number of reflections	24183
<I/σ(I)>	16.1	2.5
Completeness [%]	100.0	100
Redundancy	6.9	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		0.2 M SODIUM FORMATE AND 15-20% PEG 3350, pH 7.5