2VI7
Structure of a Putative Acetyltransferase (PA1377)from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-19 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.709, 110.928, 134.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.300 - 2.250 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ge3 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.370 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.300 | 2.310 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.090 | 0.610 |
Number of reflections | 28683 | |
<I/σ(I)> | 21 | 2.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | HANGING DROP VAPOR DIFFUSION. RESERVOIR OF 500 MICROLITRES 0.1M TRIS-HCL AT PH6.5, 1.6M AMMONIUM SULFATE AND 0.1% SODIUM AZIDE. DROPS CONTAINED 1 MICROLITRE OF PROTEIN AT 13MG /ML AND AN EQUAL VOLUME OF RESERVOIR. DROPS WERE KEPT AT 291K. |