2VHG
Crystal Structure of the ISHp608 Transposase in Complex with Right End 31-mer DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.466, 90.925, 93.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.2847 |
| Rwork | 0.285 |
| R-free | 0.31970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a6o |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.370 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.060 | 0.560 |
| Number of reflections | 13127 | |
| <I/σ(I)> | 25 | 3.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 15-20% PEG 3350 AND 0.1 M MES PH 6.5 |
