2VHD
Crystal Structure Of The Di-Haem Cytochrome C Peroxidase From Pseudomonas aeruginosa - Mixed Valence Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-08 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.200, 44.800, 106.200 |
| Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
| Resolution | 76.700 - 2.300 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c1v |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.592 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.720 |
| Number of reflections | 34786 | |
| <I/σ(I)> | 16 | 1.6 |
| Completeness [%] | 98.6 | 95.8 |
| Redundancy | 4.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 24% PEG 600, 0.2 M IMIDAZOLE MALATE PH 5.5, 20 MM DTT |
