Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-07-10 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.036, 171.795, 85.086 |
Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.740 |
R-factor | 0.254 |
Rwork | 0.253 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vgb |
RMSD bond length | 0.025 |
RMSD bond angle | 1.952 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.100 | 0.470 |
Number of reflections | 50701 | |
<I/σ(I)> | 5.5 | 2.1 |
Completeness [%] | 92.6 | 90.1 |
Redundancy | 2.1 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.4 | pH 6.4 |