2VGA
The structure of Vaccinia virus A41
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.608, 60.839, 50.407 |
Unit cell angles | 90.00, 91.04, 90.00 |
Refinement procedure
Resolution | 50.380 - 1.900 |
R-factor | 0.2 |
Rwork | 0.194 |
R-free | 0.24900 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.533 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.880 |
Number of reflections | 17533 | |
<I/σ(I)> | 25.7 | 2.4 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7.7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M POTASSIUM FLUORIDE, 20 % POLYETHYLENE GLYCOL 3350. |