2VGA
The structure of Vaccinia virus A41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.608, 60.839, 50.407 |
| Unit cell angles | 90.00, 91.04, 90.00 |
Refinement procedure
| Resolution | 50.380 - 1.900 |
| R-factor | 0.2 |
| Rwork | 0.194 |
| R-free | 0.24900 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.533 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.880 |
| Number of reflections | 17533 | |
| <I/σ(I)> | 25.7 | 2.4 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M POTASSIUM FLUORIDE, 20 % POLYETHYLENE GLYCOL 3350. |
