2VG9
Crystal structure of Loop Swap mutant of Necallimastix patriciarum Xyn11A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-02 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 117.420, 117.420, 117.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.050 - 2.000 |
| R-factor | 0.181 |
| Rwork | 0.178 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c1f |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.520 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.200 |
| Number of reflections | 27316 | |
| <I/σ(I)> | 15.3 | 6.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1 M CADMIUM CHLORIDE, 0.1 M SODIUM ACETATE, PH 4.6 AND 30% (W/V) POLYETHYLENE GLYCOL 400 |
