2VFV
Alditol Oxidase from Streptomyces coelicolor A3(2): Complex with Sulphite
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Collection date | 2007-03-15 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.561, 68.455, 57.965 |
| Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
| Resolution | 19.510 - 1.720 |
| R-factor | 0.153 |
| Rwork | 0.152 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vfr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.730 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.090 |
| Number of reflections | 43108 | |
| <I/σ(I)> | 24.9 | 10.9 |
| Completeness [%] | 98.5 | 97.1 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
