2VFR
Alditol Oxidase from Streptomyces coelicolor A3(2): Native Enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-25 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.023, 68.614, 57.977 |
Unit cell angles | 90.00, 95.15, 90.00 |
Refinement procedure
Resolution | 57.740 - 1.100 |
R-factor | 0.14 |
Rwork | 0.139 |
R-free | 0.16500 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.015 |
RMSD bond angle | 1.719 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.700 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.010 | 0.430 |
Number of reflections | 167187 | |
<I/σ(I)> | 9 | 3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |